MITCHELL LAB

UofTlogo

CSB

Welcome to the Mitchell Lab

How does our genome regulate the process of development and cell-type specialisation? Specific genes are expressed at discrete times due to the activation or repression of DNA regulatory regions. Our research investigates how the genome functions in stem cells to regulate self-renewal and differentiation. We often think about transcription as occurring on a particular gene in a linear manner whereas the nucleus is a three dimension organelle into which the genome is folded and organised. Within this folded structure DNA regulatory sequences physically contact the genes they regulate forming tissue-specific chromatin loops.

We use CRISPR Genome Editing, Molecular Biology and Cellular Imaging techniques combined with Genome-Wide Sequencing approaches and Bioinformatics analysis to investigate the mechanisms that underlie tissue-specific regulation of gene expression and genome folding. Our previous work has identified the enhancers that activate numerous genes in stem cells including a distal enhancer of the Sox2 transcription factor which is required to maintain the stem cell state. The work we do provides a deeper understanding of how our DNA makes us unique and predisposes us to particular diseases. Our work also identifies new ways to consider treating genetic diseases with CRISPR Genome or Epigenome Editing.

Mitchell Lab News

Dr Navroop Dhaliwal successfully defended her doctoral thesis on Dec 10, 2018!

Professor Mitchell was Keynote Speaker at the Asilomar Chromatin, Chromosomes, and Epigenetics Conference in Pacific Grove, California, USA. Dr Macpherson presented a talk.

Professor Mitchell, Luís Abatti and Gurdeep Singh presented their work at the 5th Canadian Conference on Epigenetics in Estérel, Québec.

Welcome to Nawrah Khader, who has started her Master's project in the Mitchell Lab.

Congratulations to Christina Ji who won the Best Poster award at the CSB Undergraduate Research Symposium for her NSERC-funded undergraduate research project!

Dr Sakthi Moorthy, Lida Langroudi and Navroop Dhaliwal presented their work at ISSCR2018.

Professor Mitchell presented her work at the Precision CRISPR Stem Cell Congress 2018, running from June 12-14 in Seattle, WA.

Congratulations to Lida Langroudi and Navroop Dhaliwal on being awarded a Medicine by Design Travel Award to present their work at ISSCR2018 in Melbourne, Australia.

Congratulations to Dr Sakthi Moorthy on being awarded an OIRM Travel Award to present her research at ISSCR2018 in Melbourne, Australia.

Dr Moorthy gave a talk on her research at the New Mexico BioInformatics, Science and Technology (NMBIST) Symposium on Genome Editing in Santa Fe, New Mexico.

Our Stem Cell Reports paper: KLF4 Nuclear Export Requires ERK Activation and Initiates Exit from Naive Pluripotency is out!

Professor Mitchell spoke at Human Genome Meeting 2018 in Yokohama, Japan.

Professor Mitchell presented a talk on her research at the Keystone conference Precision Genome Editing with Programmable Nucleases. Dr Moorthy presented a poster.

Congratulations to Gurdeep Singh on being awarded the Zoology International Award from Cell & Systems Biology.

Professor Mitchell gave a talk on her research at the 4th Canadian Conference on Epigenetics - Mechanisms of Disease. Dr Macpherson presented a poster.

Professor Mitchell has been awarded tenure!

Our Genome Research paper: Enhancers and super-enhancers have an equivalent regulatory role in embryonic stem cells through regulation of single or multiple genes is out!

Professor Jennifer Mitchell is the recipient of the inaugural Dorothy Shoichet Women Faculty in Science Award of Excellence.

Virlana Shchuka has been awarded a PGS-D fellowship from NSERC, awarded to high-calibre students who are engaged in doctoral programs in the natural sciences or engineering.

Congratulations to Virlana Shchuka on receiving the Duncan L. Gellatly Memorial Award for excellence in research.

OIRM New Ideas grant on 'Activating enhancers to improve reprogramming efficiency' awarded to the Mitchell Lab.

Mitchell Lab receives an NSERC Discovery Grant.

U of T Cell Biologists Discover On-Off Switch for Key Stem Cell Gene

Key stem cell gene controlled from afar, Canadian scientists discover

 

CIHR NSERC Ontario Research Fund CFI