Dr. Wolfgang Moeder

Current Work on Rice

We are interested in the disease resistance signaling pathway of rice. Recently, the rice genome has been sequenced, and rice is rapidly becoming a model system for monocotyledonous plants. It has been suggested that dicot and monocot plants have some differences in their defense systems, and it is important to use both model plants (Arabidopsis and rice) in this research field. In particular, since the monocot plant group includes agriculturally important crops such as wheat, barley, and corn, the analysis of the rice defense system will have a significant impact on agriculture.

As a model system we are studying the interaction of rice with the pathogen Cochliobolus miyabeanus (Bipolaris oryzae) which causes the brown spot disease. We would like to elucidate the molecular mechanism of rice resistance to this pathogen. One approach will be MicroArray analysis to identify novel components of the rice disease resistance signaling pathway. The U of T, Department of Cell and Systems Biology has a state-of-the-art facility for this analysis (http://www.csb.utoronto.ca/). In addition to the molecular analysis a detailed analysis of plant hormones will be conducted using GC-MS. The combination of molecular and physiological analyses will provide us with new insight into the rice resistance signaling pathway.

 

 

Publications

1. Urquhart, W., Gunawardena, A., Moeder, W., Ali, R., Berkowitz, G., Yoshioka, K. (2007).  The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner. Plant Mol Biol . 65(6), 747-61. Abstract

2. Yoshioka, K., Moeder, W., Kang, H., Masmoudi, P.K.K., Berkowitz, G., Klessig, D.F. (2006).  The Chimeric Arabidopsis CYCLIC NUCLEOTIDE-GATED ION CHANNEL11/12 Activates Multiple Pathogen Resistance Responses. The Plant Cell. 18, 747–763. Abstract

3. Moeder, W., Yoshioka, K., and Klessig, D.F. (2005) Involvement of the small GTPase Rac in the defense responses of tobacco to pathogens. Mol. Plant-Microbe Interact. 18, 116-124. Abstract

4. Nandi, A., Moeder, W., Kachroo, P., Klessig, D.F., and Shah, J. (2005) The Arabidopsis ssi2-conferred susceptibility to Botrytis cinerea is dependent on EDS5 and PAD4. Mol. Plant-Microbe Interact. 18(4), 363-70. Abstract

5. Zhou, F., Menke, F.L.H., Yoshioka, K., Moder, W., Shirano, Y., and Klessig, D.F. (2004) High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J. 39, 920-932.

6. Barth, C., Moeder, W., Klessig, D.F., and Conklin P.L. (2004) The timing of senescence and response to pathogens is altered in the ascorbate-deficient Arabidopsis mutant vitamin c-1. Plant Physiol. 134, 1794-1792.

7. Chen, Z., Kloek, A.P., Cuzick, A., Moeder, W. Tang, D., Innes, R., Klessig, D.F., McDowell, J.M., and Kunkel, B. (2004) The Pseudomonas syringae type III effector AvrRpt2 functions downstream or independently of SA to promote virulence on Arabidopsis thaliana. Plant J. 37, 494-504.

8. Moeder, W., Tauriainen, A., Barry, C., Betz, C., Tuomainen, J., Utriainen, M., Grierson, D., Langebartels, C., Sandermann, H., and Kangasjärvi, J. (2002) Ethylene synthesis regulated by biphasic induction of 1-aminocyclopropane-1-carboxylic acid synthase and 1-aminocyclopropane-1-carboxylic acid oxidase genes is required for hydrogen peroxide accumulation and cell death in ozone-exposed tomato. Plant Physiol. 130, 1918-1926.

9. Möder, W., Bunk, A., Albrecht, A., Doostdar, H., Niedz, R.P., McDonald, R.E., Mayer, R.T., and Osswald, W. (1999) Characterization of acidic chitinases from culture medium of sweet orange callus tissue. J. Plant Physiol. 154, 296-301.

10. Chamnongpol, S., Willekens, H., Moeder, W., Langebartels, C., Sandermann, H., Van Montagu, M., Inzé, D., and Van Camp, W. (1998) Defense activation and enhanced pathogen tolerance induced by H2O2 in transgenic tobacco. Proc. Natl. Acad. Sci. USA 95, 5818-5823.

11. Schraudner, M., Moeder, W., Wiese, C., Van Camp, W., Inzé, D., Langebartels, C., and Sandermann, H. (1998) Ozone-induced oxidative burst in the ozone biomonitor plant, tobacco Bel W3. Plant J. 16, 235-245.

 

Book Chapters

1. Moeder, W., Kangasjärvi, J., Elstner, E.F., Langebartels, C. and Sandermann, H. (1999) Apoplastic ACC in ozone- and elicitor-treated plants. In: Biology and Biotechnology of the Plant Hormone Ethylene II., A.K. Kanellis, C. Chang, H. Klee, J.C. Pech and D. Grierson, eds., Kluwer, Dordrecht, pp 345-346.

2. Moeder, W. Anegg, S., Thomas, G., Langebartels, C. and Sandermann, H. (1999) Signal molecules in ozone activation of stress proteins In: plants. In: Plant Responses to Environmental Stress. (Smallwood, M.F., Clavert, C.M., Bowles, D.J., eds.) Oxford: BIOS Scientific Publishers, pp. 43-49.